Lyme disease is caused by Borrelia burgdorferi, a spiral-shaped bacterium transmitted by deer ticks. Most cases of Lyme disease can be treated effectively with a short course of antibiotics. However, Lyme disease can be difficult to diagnose because it causes a wide range of symptoms, from fever and rash to neurologic and cardiac symptoms and joint pain. Current Lyme disease tests also can miss an infection if performed too early.
The Centers for Disease Control and Prevention recommends a two-step blood test for diagnosing Lyme disease that looks for antibodies against Lyme disease. These tests require specialized laboratory equipment and can require days or weeks to return results. The authors of the paper plan to develop a simpler, faster, more sensitive test that could be used at the point of care during a single visit to a healthcare provider.
The researchers first screened a set of known Lyme disease biomarkers for their ability to indicate infection. They then tested for the top three biomarkers on samples from people with early Lyme disease, from healthy individuals from areas where Lyme disease is endemic, and from people with Lyme arthritis. This was compared to results obtained using the standard two-step testing procedure.
Overall, the new set of biomarkers was more sensitive than standard Lyme disease tests. These biomarkers were better at picking up signs of Lyme disease infection in early-stage samples - possibly because they were able to detect antibodies that peak in the first two to six weeks after a person is infected with Lyme disease. These results open the possibility of developing a point-of-care test for Lyme disease.
While the assay will require more refinement and testing before it can be approved by the Food and Drug Administration for widespread use as a simple diagnostic test for Lyme disease, the researchers say that these results show great potential.
Arumugam et al. A multiplexed serologic test for diagnosis of Lyme disease for point-of-care use. Journal of Clinical Microbiology DOI: 10.1128/JCM.01142-19 (2019).
Adriana Marques, M.D., chief of the Lyme Disease Studies Unit in the Laboratory of Clinical Immunology & Microbiology at NIAID and an author of this study, is available for comment.
This research was supported in part by NIAID grant R44 AI096551 and in part by NIAID’s Intramural Research Program.
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